Journal: Biomedicines
Article Title: Cytogenomic Profile of Uterine Leiomyoma: In Vivo vs. In Vitro Comparison
doi: 10.3390/biomedicines9121777
Figure Lengend Snippet: Telomere detection in the metaphase chromosomes from a UL cell ( A ) and a cell of adjacent myometrium ( B ) after in vitro culturing (magnification 10 × 100, var. × 0.63). Telomeres were detected through fluorescence in situ hybridisation (FISH) with telomeric DNA probes (Telomere PNA FISH/Cy3; DAKO, Denmark) and reference locus-specific probes (Vysis LSI 13 RB1 13q14 SpectrumOrange Probe; (Abbott Laboratories, USA). The chromosomes were stained with DAPI.
Article Snippet: For simultaneous detection of telomeric regions and the 13q14 region (the reference region for telomere length measurements in our study), a mixture of telomeric (Telomere PNA FISH/Cy3; DAKO, Glostrup, Denmark) and locus-specific probes (LSI 13 RB1 13q14 SpectrumOrange Probe; Abbott Laboratories, USA) was applied.
Techniques: In Vitro, Fluorescence, In Situ, Hybridization, Staining